Journal: Journal of Nanobiotechnology

Article Title: Nanoparticle-integrated smart hydrogel with H 2 S-triggered antibacterial escalation and sustained oxygen release for periodontal regeneration

doi: 10.1186/s12951-026-04535-7

Figure Lengend Snippet: Osteogenic differentiation of hPDLSCs induced by PDA@Hb/NOCG. ( A ) Representative ALP staining results of hPDLSCs after 7 days of osteogenic differentiation. ( B ) Alizarin Red S staining of hPDLSCs after 21 days of osteogenic induction. ( C ) Quantitative analysis of ALP staining in hPDLSCs. ( D ) Quantification of Alizarin Red S staining in hPDLSCs. ( E-K ) Relative mRNA expression levels of osteogenesis-related genes in hPDLSCs, including ALP, BMP2, COL1A1, OPG, RUNX2, OPN, and OCN, normalized to GAPDH. ( L-O ) Protein expression levels of osteogenesis-related markers in hPDLSCs, including ALP, RUNX2, and β-catenin, and their corresponding quantitative analysis. ( P ) Protein expression levels of osteogenesis-related markers, including ALP, RUNX2, and β-catenin, in hPDLSCs under different treatment conditions with or without DKK1. ( Q ) Corresponding quantitative analysis of ALP, RUNX2, and β-catenin protein expression in each group

Article Snippet: Primary antibodies against ALP (rabbit polyclonal IgG, Cat. No. 18507-1-AP), NF-κB p65 (rabbit polyclonal IgG, Cat. No. 10745-1-AP), and β-catenin (rabbit polyclonal IgG, Cat. No. 17565-1-AP) were purchased from Proteintech (Wuhan, China), while the antibodies against RUNX2 (rabbit polyclonal IgG, Cat. No. HY- P80316 ) and Hb (polyclonal antibody, Cat. No. PAB409Bo01) were obtained from MedChemExpress (MCE, Shanghai, China) and Cloud-Clone Corp. (Wuhan, China), respectively.

Techniques: Staining, Expressing

Journal: Bioactive Materials

Article Title: MSC-mimicking nanovesicle embedded bio-adhesive hydrogel for dual immunomodulation and osteogenesis to promote maxillofacial bone regeneration

doi: 10.1016/j.bioactmat.2026.02.032

Figure Lengend Snippet: In vivo evaluation of bone regeneration after hydrogel implantation in the mandibular bone defect mouse model. a) Schematic illustration of the mandibular bone defect mouse model. b) Micro-CT 3D reconstruction images of the mandibular bone samples at 4 and 8 weeks post-surgery. Scale bar = 1 mm. c) Semi-quantitative analysis of BV/TV, bone surface, Tb.N and Tb.sp (n = 6) in mouse mandibular bone defects implanted with different hydrogels at 8 weeks post-surgery. d) H&E staining and Masson trichrome staining of tissue sections of mandibular defects at 8 weeks post-surgery. Scale bar = 100 μm. e, f) Immunofluorescent staining images and corresponding semi-quantitative analysis of the expression levels of RUNX2 and OCN in mandibular bone defect areas at 4 and 8 weeks post-surgery (n = 3). Scale bar = 50 μm. P-values are calculated using one-way ANOVA with Tukey's test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, n.s. not significant ( a was created with bioRender. com).

Article Snippet: Briefly, tissue sections underwent antigen retrieval (37 °C, 30 min), blocked by 5% bovine serum albumin (BSA, RT, 1 h), then sequentially incubated (4 °C) with lineage-specific probes: macrophage marker F4/80 (1:200, Cat. sc-52664, Santa Cruz Biotechnology) and CD68 (1:250, Cat. 14-0681-81, ThermoFisher, USA), CD206 (1:500, Cat. 24595T, Cell Signaling Technology, USA) and Arg-1 (1:250, Cat. 82975, Proteintech, China) for a M2 marker, iNOS (1:500, Cat. ab178945, Abcam, UK) and CD86 (1:300, Cat. DF6332, Affinity, China) for a M1 marker, RUNX2 (1:150, Cat. sc390351, Santa Cruz Biotechnology, USA) and OCN (1:150, Cat. sc390877, Santa Cruz Biotechnology, USA) for osteogenesis markers, VEGF (1:50, Cat. sc57496, Santa Cruz Biotechnology, USA) and CD31 (1:50, Cat. sc20071, Santa Cruz Biotechnology, USA) for angiogenesis markers, and CD146 (1:200, Cat. Ab75769, Abcam, UK) for stem cell surface marker, overnight at 4 °C.

Techniques: In Vivo, Micro-CT, Staining, Expressing

Journal: Bioactive Materials

Article Title: MSC-mimicking nanovesicle embedded bio-adhesive hydrogel for dual immunomodulation and osteogenesis to promote maxillofacial bone regeneration

doi: 10.1016/j.bioactmat.2026.02.032

Figure Lengend Snippet: The PEG-pp@nMSC@MT hydrogel effectively promotes BMMSCs' osteogenesis in vitro . a) Schematic illustration of co-culture BMMSCs with hydrogels. b) ALP staining of BMMSCs co-cultured with different scaffolds after 7 days. Scale bar = 500 μm. c) Semi-quantitative analysis of ALP staining (n = 3). d) ARS staining of BMMSC co-cultured with different scaffolds after 21 days. Scale bar = 500 μm. e) Semi-quantitative analysis of ARS staining (n = 3). f) mRNA expression of osteogenic genes (BMP2, OCN, and RUNX2) of BMMSCs treated with different hydrogels in the MMP condition after 5 and 10 days (n = 3). g) Western blot analysis of osteogenic protein (BMP2 and RUNX2) of BMMSCs treated with different hydrogels in the MMP condition after 7 and 14 days. P-values are calculated using one-way ANOVA with Tukey's test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, n.s. not significant ( a was created with bioRender. com).

Article Snippet: Briefly, tissue sections underwent antigen retrieval (37 °C, 30 min), blocked by 5% bovine serum albumin (BSA, RT, 1 h), then sequentially incubated (4 °C) with lineage-specific probes: macrophage marker F4/80 (1:200, Cat. sc-52664, Santa Cruz Biotechnology) and CD68 (1:250, Cat. 14-0681-81, ThermoFisher, USA), CD206 (1:500, Cat. 24595T, Cell Signaling Technology, USA) and Arg-1 (1:250, Cat. 82975, Proteintech, China) for a M2 marker, iNOS (1:500, Cat. ab178945, Abcam, UK) and CD86 (1:300, Cat. DF6332, Affinity, China) for a M1 marker, RUNX2 (1:150, Cat. sc390351, Santa Cruz Biotechnology, USA) and OCN (1:150, Cat. sc390877, Santa Cruz Biotechnology, USA) for osteogenesis markers, VEGF (1:50, Cat. sc57496, Santa Cruz Biotechnology, USA) and CD31 (1:50, Cat. sc20071, Santa Cruz Biotechnology, USA) for angiogenesis markers, and CD146 (1:200, Cat. Ab75769, Abcam, UK) for stem cell surface marker, overnight at 4 °C.

Techniques: In Vitro, Co-Culture Assay, Staining, Cell Culture, Expressing, Western Blot

Journal: JBMR Plus

Article Title: Follistatin-like protein 1 (FSTL1) modulates bone remodeling and attenuates bone loss in a mouse model of postmenopausal osteoporosis

doi: 10.1093/jbmrpl/ziag037

Figure Lengend Snippet: Fstl1 enhances the osteogenic differentiation potential of MC3T3-E1 cells. (A) Representative alkaline-phosphatase (ALP) staining at 7 d. (B) Quantitative ALP activity. (C) Representative Alizarin Red S (ARS) staining at 14 d. (D) Quantification of ARS absorbance (OD 562 ). (E-H) Relative mRNA expression of Runx2, Sp7, Ocn, and Opn at 14 d determined by RT-qPCR. (I) Western-blot bands for RUNX2 and SP7. (J and K) Densitometric ratios of RUNX2/GAPDH and SP7/GAPDH. Sample sizes: con ( n = 3), OE-Fstl1 ( n = 3), and sh-Fstl1 ( n = 3). Data are presented as mean ± SD; inter-group comparisons were performed using 1-way ANOVA. * p < .05, ** p < .01, *** p < .001, **** p < .0001; ns, not significant.

Article Snippet: Antibodies against RUNX2, SP7, NFATC1, and CTSK were purchased from Abcam, while the anti-GAPDH antibody and anti-FSTL1 antibody were obtained from Proteintech.

Techniques: Staining, Activity Assay, Expressing, Quantitative RT-PCR, Western Blot

Journal: Cureus

Article Title: High Immunohistochemical Expression of Runt-Related Transcription Factor 2 (RUNX2) Is Associated With High Tumor Grade, Muscle Invasion, Lymph Node Metastasis, and Advanced Stage in Urinary Bladder Cancer

doi: 10.7759/cureus.106976

Figure Lengend Snippet: IHC: immunohistochemistry; H&E: hematoxylin and eosin; RUNX2: Runt-related transcription factor 2.

Article Snippet: Immunohistochemistry (IHC) was then performed using the commercially available rabbit polyclonal RUNX2 antibody (Clone: Ab-AF5186#1486, pack size 100 μl, concentration of 1 mg/ml) from Affinity Biosciences, Cincinnati, OH, USA.

Techniques: Immunohistochemistry

Journal: Cureus

Article Title: High Immunohistochemical Expression of Runt-Related Transcription Factor 2 (RUNX2) Is Associated With High Tumor Grade, Muscle Invasion, Lymph Node Metastasis, and Advanced Stage in Urinary Bladder Cancer

doi: 10.7759/cureus.106976

Figure Lengend Snippet: IHC: immunohistochemistry; H&E: hematoxylin and eosin; RUNX2: Runt-related transcription factor 2.

Article Snippet: Immunohistochemistry (IHC) was then performed using the commercially available rabbit polyclonal RUNX2 antibody (Clone: Ab-AF5186#1486, pack size 100 μl, concentration of 1 mg/ml) from Affinity Biosciences, Cincinnati, OH, USA.

Techniques: Immunohistochemistry

Journal: Cureus

Article Title: High Immunohistochemical Expression of Runt-Related Transcription Factor 2 (RUNX2) Is Associated With High Tumor Grade, Muscle Invasion, Lymph Node Metastasis, and Advanced Stage in Urinary Bladder Cancer

doi: 10.7759/cureus.106976

Figure Lengend Snippet: IHC: immunohistochemistry; H&E: hematoxylin and eosin; RUNX2: Runt-related transcription factor 2.

Article Snippet: Immunohistochemistry (IHC) was then performed using the commercially available rabbit polyclonal RUNX2 antibody (Clone: Ab-AF5186#1486, pack size 100 μl, concentration of 1 mg/ml) from Affinity Biosciences, Cincinnati, OH, USA.

Techniques: Immunohistochemistry

Journal: Dose-Response

Article Title: Skeletal Stem Cells Rescue Radiation-Induced Osteogenic Precursor Cell Dysfunction via the Wnt/β-Catenin Signaling Pathway

doi: 10.1177/15593258261440983

Figure Lengend Snippet: SSCs exert rescue effects via the Wnt/β-catenin signaling pathway. (A) Representative ALP staining images of cells in each group (Scale bar: 50 μm). (B) Quantitative analysis of ALP activity in each group. (C) Representative Alizarin Red S staining images of cells in each group (Scale bar: 100 μm). (D) Quantitative analysis of Alizarin Red S staining in each group. (E) Relative mRNA expression levels of osteogenic marker genes ( Runx2 , Col1a1 , and OCN ) detected by qRT-PCR. GAPDH was used as an internal reference gene. (F) Representative Western blot images showing the expression levels of RUNX2, COL1A1, OCN, and β-catenin in each group. GAPDH was used as a loading control. (G) Quantitative analysis of Western blot results (gray value ratio of target protein to GAPDH) in each group. All data are presented as mean ± SD, with statistical significance determined by unpaired two-tailed Student’s t-test (* p < 0.05; ** p < 0.01; *** p < 0.001).

Article Snippet: Membranes were blocked with 5% skim milk for 1 hour at room temperature, and then incubated with primary antibodies targeting RUNX2 (1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat. No. 12556), COL1A1 (1:1000, Cell Signaling Technology, Cat. No. 72026), OCN (1:1000, Cell Signaling Technology, Cat. No. 59757), β-catenin (1:1000, Cell Signaling Technology, Cat. No. 8480), and GAPDH (1:1000, Cell Signaling Technology, Cat. No. 2118) at 4°C overnight.

Techniques: Staining, Activity Assay, Expressing, Marker, Quantitative RT-PCR, Western Blot, Control, Two Tailed Test